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Photothermal performance and in vitro antitumor efficacy of APM. (A) Temperature change profiles of APM at various concentrations, (B) at various power densities of NIR, and (C) under 4 irradiation/cooling cycles. (D) Temperature–time curves related to photothermal conversion efficiency, along with the linear relationship curves (between time and −ln △θ) of APM under 1 W/cm 2 808-nm NIR. (E) Photothermal images of PBS, AC, and APM. (F) Cell viability with 4T1 cells with various treatments (with the AC concentration of 40 μg/ml). (G) <t>Fluorescent</t> images of live/dead staining of various treatments. Scale bar, 200 μm. (H) The apoptosis rate of 4T1 cells of various treatments was analyzed by FCM and (I) the quantitative evaluation. (J) 4T1 cell invasion rate of various treatments in scratch wound healing assay and (K) the quantitative analysis. (L) 4T1 cell migration counts of various treatments in transwell assay and (M) the quantitative analysis. Data are shown as mean ± SD ( n = 3) (G1: control, G2: PDA + NIR, G3: AC, G4: AP, G5: APM, G6: APM + NIR, Chol: cholesterol).
Cell Membrane Green Fluorescent Probe Dio Dio, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Photothermal performance and in vitro antitumor efficacy of APM. (A) Temperature change profiles of APM at various concentrations, (B) at various power densities of NIR, and (C) under 4 irradiation/cooling cycles. (D) Temperature–time curves related to photothermal conversion efficiency, along with the linear relationship curves (between time and −ln △θ) of APM under 1 W/cm 2 808-nm NIR. (E) Photothermal images of PBS, AC, and APM. (F) Cell viability with 4T1 cells with various treatments (with the AC concentration of 40 μg/ml). (G) <t>Fluorescent</t> images of live/dead staining of various treatments. Scale bar, 200 μm. (H) The apoptosis rate of 4T1 cells of various treatments was analyzed by FCM and (I) the quantitative evaluation. (J) 4T1 cell invasion rate of various treatments in scratch wound healing assay and (K) the quantitative analysis. (L) 4T1 cell migration counts of various treatments in transwell assay and (M) the quantitative analysis. Data are shown as mean ± SD ( n = 3) (G1: control, G2: PDA + NIR, G3: AC, G4: AP, G5: APM, G6: APM + NIR, Chol: cholesterol).
Dio (Cell Membrane Green Fluorescent Probe), supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beyotime dio green fluorescent probe for cell membranes c1038
Photothermal performance and in vitro antitumor efficacy of APM. (A) Temperature change profiles of APM at various concentrations, (B) at various power densities of NIR, and (C) under 4 irradiation/cooling cycles. (D) Temperature–time curves related to photothermal conversion efficiency, along with the linear relationship curves (between time and −ln △θ) of APM under 1 W/cm 2 808-nm NIR. (E) Photothermal images of PBS, AC, and APM. (F) Cell viability with 4T1 cells with various treatments (with the AC concentration of 40 μg/ml). (G) <t>Fluorescent</t> images of live/dead staining of various treatments. Scale bar, 200 μm. (H) The apoptosis rate of 4T1 cells of various treatments was analyzed by FCM and (I) the quantitative evaluation. (J) 4T1 cell invasion rate of various treatments in scratch wound healing assay and (K) the quantitative analysis. (L) 4T1 cell migration counts of various treatments in transwell assay and (M) the quantitative analysis. Data are shown as mean ± SD ( n = 3) (G1: control, G2: PDA + NIR, G3: AC, G4: AP, G5: APM, G6: APM + NIR, Chol: cholesterol).
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Photothermal performance and in vitro antitumor efficacy of APM. (A) Temperature change profiles of APM at various concentrations, (B) at various power densities of NIR, and (C) under 4 irradiation/cooling cycles. (D) Temperature–time curves related to photothermal conversion efficiency, along with the linear relationship curves (between time and −ln △θ) of APM under 1 W/cm 2 808-nm NIR. (E) Photothermal images of PBS, AC, and APM. (F) Cell viability with 4T1 cells with various treatments (with the AC concentration of 40 μg/ml). (G) <t>Fluorescent</t> images of live/dead staining of various treatments. Scale bar, 200 μm. (H) The apoptosis rate of 4T1 cells of various treatments was analyzed by FCM and (I) the quantitative evaluation. (J) 4T1 cell invasion rate of various treatments in scratch wound healing assay and (K) the quantitative analysis. (L) 4T1 cell migration counts of various treatments in transwell assay and (M) the quantitative analysis. Data are shown as mean ± SD ( n = 3) (G1: control, G2: PDA + NIR, G3: AC, G4: AP, G5: APM, G6: APM + NIR, Chol: cholesterol).
Cell Membrane Green Fluorescent Probe Dio Beyotime, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Yeasen Biotechnology dio cell membrane green fluorescent probe saturated solution
Photothermal performance and in vitro antitumor efficacy of APM. (A) Temperature change profiles of APM at various concentrations, (B) at various power densities of NIR, and (C) under 4 irradiation/cooling cycles. (D) Temperature–time curves related to photothermal conversion efficiency, along with the linear relationship curves (between time and −ln △θ) of APM under 1 W/cm 2 808-nm NIR. (E) Photothermal images of PBS, AC, and APM. (F) Cell viability with 4T1 cells with various treatments (with the AC concentration of 40 μg/ml). (G) <t>Fluorescent</t> images of live/dead staining of various treatments. Scale bar, 200 μm. (H) The apoptosis rate of 4T1 cells of various treatments was analyzed by FCM and (I) the quantitative evaluation. (J) 4T1 cell invasion rate of various treatments in scratch wound healing assay and (K) the quantitative analysis. (L) 4T1 cell migration counts of various treatments in transwell assay and (M) the quantitative analysis. Data are shown as mean ± SD ( n = 3) (G1: control, G2: PDA + NIR, G3: AC, G4: AP, G5: APM, G6: APM + NIR, Chol: cholesterol).
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Photothermal performance and in vitro antitumor efficacy of APM. (A) Temperature change profiles of APM at various concentrations, (B) at various power densities of NIR, and (C) under 4 irradiation/cooling cycles. (D) Temperature–time curves related to photothermal conversion efficiency, along with the linear relationship curves (between time and −ln △θ) of APM under 1 W/cm 2 808-nm NIR. (E) Photothermal images of PBS, AC, and APM. (F) Cell viability with 4T1 cells with various treatments (with the AC concentration of 40 μg/ml). (G) Fluorescent images of live/dead staining of various treatments. Scale bar, 200 μm. (H) The apoptosis rate of 4T1 cells of various treatments was analyzed by FCM and (I) the quantitative evaluation. (J) 4T1 cell invasion rate of various treatments in scratch wound healing assay and (K) the quantitative analysis. (L) 4T1 cell migration counts of various treatments in transwell assay and (M) the quantitative analysis. Data are shown as mean ± SD ( n = 3) (G1: control, G2: PDA + NIR, G3: AC, G4: AP, G5: APM, G6: APM + NIR, Chol: cholesterol).

Journal: Biomaterials Research

Article Title: Biomimetic Atorvastatin Self-Assembled Nanomedicine Inhibits the Cyclooxygenase-2/Prostaglandin E2 Pathway Enhanced Photothermal and Antitumor Immunity

doi: 10.34133/bmr.0149

Figure Lengend Snippet: Photothermal performance and in vitro antitumor efficacy of APM. (A) Temperature change profiles of APM at various concentrations, (B) at various power densities of NIR, and (C) under 4 irradiation/cooling cycles. (D) Temperature–time curves related to photothermal conversion efficiency, along with the linear relationship curves (between time and −ln △θ) of APM under 1 W/cm 2 808-nm NIR. (E) Photothermal images of PBS, AC, and APM. (F) Cell viability with 4T1 cells with various treatments (with the AC concentration of 40 μg/ml). (G) Fluorescent images of live/dead staining of various treatments. Scale bar, 200 μm. (H) The apoptosis rate of 4T1 cells of various treatments was analyzed by FCM and (I) the quantitative evaluation. (J) 4T1 cell invasion rate of various treatments in scratch wound healing assay and (K) the quantitative analysis. (L) 4T1 cell migration counts of various treatments in transwell assay and (M) the quantitative analysis. Data are shown as mean ± SD ( n = 3) (G1: control, G2: PDA + NIR, G3: AC, G4: AP, G5: APM, G6: APM + NIR, Chol: cholesterol).

Article Snippet: Cell Membrane Green Fluorescent Probe DiO (DiO) was obtained from Beyotime Biotechnology (China).

Techniques: In Vitro, Irradiation, Concentration Assay, Staining, Wound Healing Assay, Migration, Transwell Assay, Control